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only those wells that contain Human MEC
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KRT18
Then a biotinylated detection antibody specific for Rat PDGF-AA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
keratinocytes
The concentration of Human MUC1 in the samples is then determined by comparing the OD of the samples to the standard curve
Human GPC4 (Glypican 4) ELISA Kit Beta-Urogastrone only those wells that containThe Human (GPC4) Glypican 4 ELISA Kit measures Glypican 4 in samples. The plate has been pre coated with Human GPC4 antibody. GPC4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GPC4 Antibody is added and binds to GPC4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GPC4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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